Chaga Antioxidant Findings
for full article: http://www.jstage.jst.go.jp/article/cpb/55/8/1222/_pdf
Antioxidant Small Phenolic Ingredients in Inonotus obliquus (persoon) Pilat (Chaga)
1) Niigata University of Pharmacy and Applied Life Science
Yuki Nakajima1), Yuzo Sato1) and Tetsuya Konishi1
(Received April 12, 2007)
(Accepted June 1, 2007))
Inonotus obliquus (persoon) Pilat (Chaga, in Russia, kabanoanatake in Japan) is a fungus having been used as a folk medicine in Russia and said to have many health beneficial functions such as immune modulating and anti-cancer activities. In the present study, the antioxidant activity of hot water extract (decoction) of Chaga was precisely compared with those of other medicinal fungi (Agaricus blazei Mycelia, Ganoderma lucidum and Phellinus linteus) showing Chaga had the strongest antioxidant activity among fungi examined in terms of both superoxide and hydroxyl radicals scavenging activities. Further determination of the antioxidant potential of isolated fruiting body (brown part) and Sclerotium (black part) revealed the 80% MeOH extract of fruiting body had the highest potential as high as that of Chaga decoction. Finally, seven antioxidant components were isolated and purified from the 80% MeOH extract of Chaga fruiting body, and their chemical structures were determined as small phenolics as follows: 4-hydroxy-3,5-dimethoxy benzoic acid 2-hydroxy-1-hydroxymethyl ethyl ester (BAEE), protocatechic acid (PCA), caffeic acid (CA), 3,4-dihybenzaladehyde (DB), 2,5-dihydroxyterephtalic acid (DTA), syringic acid (SA) and 3,4-dihydroxybenzalacetone (DBL). Notably, BAEE was assigned as the new compound firstly identified from the natural source in the present study
Chaga and Novel Platelet Aggregation Findings
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Isolation and characterization of a novel platelet aggregation inhibitory peptide from the medicinal mushroom, Inonotus obliquus
Kwang Wook Hyuna, Seung Chan Jeonga, Dae Hyoung Leea, Jeong Sik Parkb and Jong Soo Leea, 
, 
Department of Genetic Engineering and Bio-Medicinal Resources Research Center, Paichai University, Daejeon 302-735, Republic of Korea
National Institute of Agricultural Science and Technology, Suwon 441-707, Republic of Korea
Received 27 July 2005;
revised 6 October 2005;
accepted 6 October 2005.
Abstract
This study describes the extraction and characterization of a platelet aggregation inhibitory peptide from Inonotus obliquus. Ethanol extract from I. obliquus ASI 74006 mycelia showed the highest platelet aggregation inhibitory activity (81.2%). The maximum platelet aggregation inhibitory activity was found when the mycelia of I. obliquus ASI 74006 was extracted with ethanol at 80 °C for 12 h. The platelet aggregation inhibitor was purified by systematic solvent fractionation, ultrafiltration, Sephadex G-10 column chromatography, and reverse-phase HPLC. The purified platelet aggregation inhibitor is a novel tripeptide with a molecular mass of 365 Da, having a sequence of Trp-Gly-Cys. The purified platelet aggregation inhibitor also showed high platelet aggregation inhibitory activity in Institute of Cancer Research (ICR) mice.
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Identification of Inonotus obliquus (Chaga) and Analysis of Antioxidation and Antitumor Activities of Polysaccharides – Yana Song et.al.
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PDF with background on extraction processes
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Chaga Literature
Mizuno T. (1999). “Antitumor and hypoglycemic activities of polysaccharides from the sclerotia and mycelia of Inonotus obliquus”. International Journal of Medicinal Mushrooms1 (1): 301–316.
Smith JE, Rowan NJ, Sullivan R Medicinal Mushrooms: Their Therapeutic Properties and Current Medical Usage with Special Emphasis on Cancer Treatments, 2001
Cristina Lull, Harry J. Wichers, and Huub F. J. Savelkoul “Antiinflammatory and Immunomodulating Properties of Fungal Metabolites”, Wageningen University and Research Center, The Netherlands 2005
Ulrike Lindequist, Timo H. J. Niedermeyer, and Wolf-Dieter Jülich ” The Pharmacological Potential of Mushrooms”, Oxford University Press 2005
Andrea T. Borchers, Anita Krishnamurthy, Carl L. Keen, Frederick J. Meyersà , and M. Eric Gershwin “Immunobiology of Mushrooms”, Society for Experimental Biology and Medicine 2008